ࡱ> NPM#` RLbjbj2T.......B $ Bp@   h#l].kkk.. *%%%k. . %k%%..% pؠ  k%@0p%##%%#. :% pkkkkBBBD$BBBBBB...... ؚHemvr1lKm[uir7h,g-N4Yb[{VT]fWvSm^ \O~[ sSfZ sc }vZZ ؚς fYOo`www.hg17.com 0sQ.͋0 4Yb[{V]KQfWr1l,ؚSmv@Fm?\ 000Abstract0 AIM: To establish a determination method of concentrations in human biologic sample following intravenous administration of cefotaxime and sulbactam. METHODS: A RPHPLC method was developed and validated to determine cefotaxime and sulbactam in plasma and urine.  HYPERLINK "http://www.studa.net/English/" The mobile phase of cefotaxime and sulbactam consisted of 1 g/L triethylamine (regulated with phosphate acid to pH=6.2) and methanol in a 686"32 and 846"16 (v/v) respectively and pumped at a flow rate of 1 mL/min. The plasma samples were deproteinated by addition of methanol, the urine samples were diluted with super pure water, then 20 L of supernatant was injected onto a C18 column and monitored with UV detector at 254 nm and 220 nm respectively. RESULTS: The assay of cefotaxime in human plasma and urine were linear over the range of 5.3-530.0 mg/L and 5.45-545.00 mg/L respectively, with the detection limit of 1 mg/L and 0.25 mg/L respectively. The assay of sulbactam in human plasma and urine were linear over the range of 1.04-208.00 mg/L, with the detection limit of 0.50 mg/L and 0.25 mg/L respectively. The average recovery of the method was (89-110)%, and the RSD within and between days were all less than 5%. The cefotaxime and sulbactam sample of plasma and urine stored in a -20! refrigerator became stable within 3 d. CONCLUSION: The assay method is rapid, accurate and usable for the determination of cefotaxime and sulbactam concentrations in plasma and urine. 000Keywords0 cefotaxime; sulbactam; chromatography, high pressure liquid; plasma; urine 000Xd0 vv0:NYnl\(u4Yb[{V]fWT(W@FmT?\m-NvoirSm^ HYPERLINK "http://www.studa.net/" RgcOhKm HYPERLINK "http://www.studa.cn/" el. el0Ǒ(uHPLCKm[4Yb[{V@FmT?\m-NSm^. (W@7h-NReQ2ulmˆ}vT ?\7h(u~4lzʑTS Nnmۏ7h r1RgagN: NC18Svg\ORggg)n[)nAm 1 mL/min4Yb[{VT]fWvAmRvR+R:N1g/Lv NYN4lnm(uxxpHBHJN "NP`bŷߥҥߌ~~ߌ~ߥҥߥҥߌߌ~ߌ~ߌ߷ߌߥh$]CJOJQJ^JaJh$]CJaJo(h$]B*CJOJQJaJph333"jh$]CJOJQJUaJo(h$]0JCJOJQJaJhrCJOJQJaJo(h$]CJOJQJaJo(h$]CJOJQJaJh$]0JOJQJo(h$]0JOJQJ14^*J\ f""4###$333L5dJ-DM gd$]$dJ-DM a$gdrL "JZ<>rt~ (0@3B33333L5d5f5ҷҮҮҮҮҷҞҍv,jh$]h$]KHOJQJU^JaJo( h$]h$]KHOJQJ^JaJUh$]CJOJQJ^JaJh$]CJaJo(h$]0JCJOJQJaJh$]CJOJQJaJo(h$]CJOJQJaJh$]B*CJOJQJaJph333"jh$]CJOJQJUaJo((04T208 mg/L cgq@Fm7hT6RYel6RY (W Nr1agN N ۏ7h20 L Km[[gqT\by. Nr1\byA []fWSm^Cmg/L \ORCg~'`VR_(Cg͑:N1/C2 V4) Bl_~'`e z:NA=1681.319C+779.655, R2=0.9996. ~'`V:N1.04^208 mg/Ln=7 . @Fm-N]fWvgNOhQSm^ cOjVke"3 :N0.5 mg/L. N?\7h-N]fWr1\byA [Sm^Cmg/L \ORCg~'`VR_(Cg͑:N1/C2) Bl_~'`e z:NA=5415.424C+1753.765, R2=0.9994 ~'`V:N1.04^208 mg/Ln=7 . ?\7h-N]fWvgNOhQSm^ cOjVke"3 :N0.25 mg/L. 002.3cSV6esR+R(u2uM6R4Yb[{V[gqTnmT]fW[gqTnm Te6RYv TSm^v@7hT?\7hv^OlYt R+Rۏ7hRg N@7hT?\7h-Noir\byN[gqT\byken3z['`Ջ\NO0-N0ؚ10.6, 106T424 mg/L 3*NSm^v4Yb[{V@Fm7hTT?\m7hTR+RnN-20!Q{Q1, 2, 3 dS\NO0-N0ؚ10.9, 109T436 mg/L 3*NSm^v4Yb[{V?\m7hT Tl>en cMRelKm[4Yb[{VSm^SS @Fm-NRSDR+R:N2.51%, 1.46%T2.41% ?\m-NRSDR+R:N0.52%, 2.39%T1.70%. hf4Yb[{V@FmT?\m7hTnN-20!Q{3 dQ3z[. 002.5.2]fW@FmT?\m7hT-20!>en3z['`Ջ\NO0-N0ؚ2.08, 10.4T104 mg/L 3*NSm^v]fW@Fm7hTT?\m7hTR+RnN-20!Q{Q1, 2, 3dS\NO0-N0ؚ2.08, 10.4T104 mg/L 3*NSm^v]fW?\m7hT Tl>en cMRelKm[]fWSm^SS @Fm-NRSDR+R:N10.33%, 4.73%T8.80% ?\m-NRSDR+R:N0.67%, 0.66%T0.76%. hf]fW@FmT?\m7hTnN-20!Q{3 dQ3z[. 003 00bNǑ(uHPLClKm[uir7h,g-N4Yb[{VT]fWSm^, T HYPERLINK "http://book.studa.com/" e.sbSv+}Yl;3=0_uirl;4-5=THPLCl;6-7=vk,7hTYt{US,Km[ew,elQnx|[,3z['`ؚ,ToNRRf[T4N^oirSm^vKm HYPERLINK "http://soft.studa.com" ^(u. 00@7hYtelSǑ(u N/lx0ؚ/lxZPˆ}vlmBR ˆ}vlm[hQ FOx'`:_ 4Yb[{VT]fWRP[-N+T goW0pWI{~g,nm-NvxPSُNWVv4l ~Ջ(uُNel7hT-N4Yb[{VT]fWGWhs N3z[ +Tϑ'YE^MNO. Ǒ(u2uvclmˆ}v, lmy_TvcS NnmKm[,O7hTYtek'Y'Y{S,Hego}Y V6esؚ. 00AmRv*gǑ(ufb_b~vfvQvnmTNfb>\,EeNpH6.20.1\O:N4lvvpH>">$>>>?? ? ?AAAAAA0B2BxBzB~BB\D`Dְְְְh$]h$]KHaJ"h$]h$]5KHOJQJ\^J#h$]h$]KHOJQJ^JaJo( h$]h$]KHOJQJ^JaJ)h$]h$]B*KHOJQJ^JaJph333,jh$]h$]KHOJQJU^JaJo(#h$]h$]KHOJQJ^JaJo(,L59==ZDDnEFXIKLLLL$dJd-D1$M [$\$a$gd$]$dJdd-D1$M [$\$a$gd$]$dJ-D1$M a$gd$] `DbDDDDDDjKlKKKKKKKLLLLLL譜s^s^Vh$]h$]o()h$]h$]B*KHOJQJ^JaJph333#h$]h$]KHOJQJ^JaJo(,jh$]h$]KHOJQJU^JaJo( h$]h$]KHOJQJ^JaJ"h$]h$]5KHOJQJ\^J+h$]h$]5B*KHOJQJ\^Jph333%h$]h$]5KHOJQJ\^Jo(.jh$]h$]5KHOJQJU\^Jo(0182P. 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